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KMID : 0613820030130060871
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Journal of Life Science
2003 Volume.13 No. 6 p.871 ~ p.878
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Isolation and Identification of a Marine Bacterium, Pseudomonas sp. BK1 Producing Extracellular Enzymes Capable of Decomposing Multiple Complex Polysaccharides
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Kim Beom-Kyu
Jeon Beong-Sam
Cha Jae-Young
Park Jeong-Won
Kim Sam-Woong
Choi Yong-Lark
Kim Ji-Yoon
Cho Young-Su
Song Jae-Young
Lee Young-Chun
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Abstract
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A marine bacterium (strain BK1) that produces extracellular enzymes capable of decomposing complex polysaccharides, such as agar, chitin, carboxymethylcellulose, xylan and mannan, was isolated from the marine red alga Porphyra dentata. Strain BK1 was gram-negative, aerobic, catalase- and oxidase-positive, polarly flagellated bacilli that produce gelatinase and urease, but not decarboxylases. The G+C content of the DNA was 51.6 mol%. The major isoprenoid quinone component was identified as an ubiquinone-8, and the major cellular fatty acids were C16:0, C16:1 w6c and C18:1 w7c. Comparative 16S rRNA sequence analysis placed strain BK1 with members of the genus Pseudomonas. On the basis of phenotypic and genotypic data, the strain BK1 was shown to be a member of the subgroup of Pseudomonas, and named as Pseudomonas sp. BK1.
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KEYWORD
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Polysaccharides, Porphyra, Pseudomonas
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